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Chat Online2009 4 8 Previous results showed that in short term NaCl treated beans increased leaf abscisic acid ABA concentration was triggered by Na but not by Cl In this work the specificity of ABA signaling for Na homeostasis was studied by comparing the plant s responses to solutions that modified accumulation of ABA and/or Na uptake and distribution such as supplemental Ca2 increased
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Chat Online2014 6 11 After grinding with mortar and pestle the leaf material was extracted with 1.2 ml of methanol containing 24 ng of 9 10 D 2 9 10 dihydrojasmonic acid 24 ng D 4 salicylic acid Sigma Aldrich 24 ng D 6 abscisic acid Santa Cruz Biotechnology Santa Cruz U.S.A and 4.8 ng of JA 13 C 6 Ile conjugate as internal standards JA 13 C 6 Ile
Chat Online2021 10 15 The results showed that the probe had excellent sensitivity and selectivity for ABA within the range of 0.100 ng/mL−150 ng/mL with an LOD of 30.0 ng/L Finally the probe was used to estimate the concentration of ABA in rice seeds and the results were similar to those obtained from the standard HPLC MS method.
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Chat Online2021 7 7 ABA induced leaf senescence involves activation of ABA biosynthesis genes such as NCEDs ABA2 AAO3 and ABA signaling genes such as ABI5 ABF2 and ABF3 18 19 20 21 22.
Chat Online2019 7 10 Salinity is a significant environmental stress factor limiting crops productivity Barley Hordeum vulgare L has a natural tolerance to salinity stress making it an interesting study object in stress biology research In the present study for the first time the effect of salinity stress on barley inflorescence developmental stages was investigated.
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Chat Online2017 2 21 The aim of this study was to examine the effect of abscisic acid ABA sucrose and auxin on grape fruit development and to assess the mechanism of these three factors on the grape fruit ripening process Different concentrations of ABA sucrose and auxin were used to treat the grape fruit and the ripening related indices such as physiological and molecular level parameters were analyzed
Chat Online2017 9 1 Endogenous ABA was analyzed with gas chromatography–mass spectrometry GC–MS 21 22 Ground plant samples were mixed with 30 mL of extraction solution containing 95 isopropanol 5 glacial acetic acid and 20 ng of ABA internal standard ± –3 5 5 7 7 7–d6–ABA .
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Chat OnlineMeasurement of Endogenous ABA in the Epidermal Strips Extraction of Epidermal Strips Fifty to 60 strips about 20 mg dry weight were incubated 4 h for each treatment and then plunged into liquid N2 lyophilized weighed and homogenized for 2 min with 10 ml methanol H20 80 20 in a motor driven tube and pestle tissue grinder 40 ml capacity .
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Chat Online2014 6 11 After grinding with mortar and pestle the leaf material was extracted with 1.2 ml of methanol containing 24 ng of 9 10 D 2 9 10 dihydrojasmonic acid 24 ng D 4 salicylic acid Sigma Aldrich 24 ng D 6 abscisic acid Santa Cruz Biotechnology Santa Cruz U.S.A and 4.8 ng of JA 13 C 6 Ile conjugate as internal standards JA 13 C 6 Ile
Chat Online2021 8 13 of 2mg mL−1 1mg mL−1 2mg mL−1 2mg mL−1 and 1mg mL−1 respectively.Aseriesofmixedstocksolutions werecomposedofmuscone 0.4–100µg mL−1 ethylpal mitate 0.08–20µg mL−1 ethyl oleate 0.08–20µg mL−1 andethylparaben 0.16–40µg mL−1 .en itwasusedto prepare for calibration curve
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Chat OnlinePlant Physiology 129 1507 1520 Kim DH Eu YJ Yoo CM Kim YW Pih KT Jin JB Kim SJ Stenmark H Hwang I 2001 Trafficking of phosphatidylinositol 3 phosphate from the trans Golgi network to the lumen of the central vacuole in plant
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Chat Online2007 12 4 Drought the most prominent threat to agricultural production worldwide accelerates leaf senescence leading to a decrease in canopy size loss in photosynthesis and reduced yields On the basis of the assumption that senescence is a type of cell death program that could be inappropriately activated during drought we hypothesized that it may be possible to enhance drought tolerance by
Chat Online2021 6 21 As an important cash crop the yield of peanut is influenced by soil acidification and pathogen infection Receptor like protein kinases play important roles in plant growth development and stress responses However little is known about the number location structure molecular phylogeny and expression of RLKs in peanut and no comprehensive analysis of RLKs in the Al stress response in
Chat Online2021 7 1 Gladiolus corms were extracted by grinding tissue in liquid nitrogen before adding 1 ml of extraction buffer 25 mM HEPES KOH pH 7.3 5 mM ethylenediamine tetraacetic acid
Chat Online2015 6 11 ABA and 2 H 6 ABA were quantified on the SRM m/z 263/153 and m/z 269/157 by using the signal of the internal standard combined with a calibration curve for ABA with /− ABA 99 as purchased from Sigma Aldrich In both cases the parent ion was selected using a 2 Da isolation width and fragmented with 30 of normalized collision energy.
Chat Online2021 7 7 CCCH is a subfamily of zinc finger proteins involved in plant growth development and stresses response The function of CCCH in regulating leaf senescence especially its roles in
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Chat Online2021 1 9 Abscisic acid ABA plays an important role in plant growth and adaptation through the ABA signaling pathway The ABA responsive element binding AREB/ABF family transcriptional factors are central regulators that integrate ABA signaling with various signaling pathways It has long been known that ABA inhibits rhizobial infection and nodule formation in legumes but the underlying molecular
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Chat Online2009 4 8 Previous results showed that in short term NaCl treated beans increased leaf abscisic acid ABA concentration was triggered by Na but not by Cl In this work the specificity of ABA signaling for Na homeostasis was studied by comparing the plant s responses to solutions that modified accumulation of ABA and/or Na uptake and distribution such as supplemental Ca2 increased
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Chat Online2021 3 19 To examine ABA dependent expression patterns of OsGRP3 seedlings were transferred to 50 ml tubes containing 100 μM ABA solution Sigma USA For drought treatments 3 week old rice plants were air dried at room temperature Seedlings were harvested at indicated time points after ABA and drought treatment for RNA extraction.
Chat Online2014 2 12 APX was extracted by grinding leaves 0.5 g in a mortar with pestle in 5 mL of 50 m m phosphate buffer pH 7.0 containing 1 m m ascorbic acid and 1 m m EDTA at 4 C followed by centrifugation as above The supernatant was used for determination of APX Zhang et al 2009 One unit of SOD activity was defined as the amount of enzyme
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